Characterization of melanoidin formed from glucose with alanine  by Fluorescence spectroscopy and redox chemistry in vitro cells

https://doi.org/10.26389/AJSRP.G120919

Authors

  • Ghassan Faisal Mohsin
  • Abdulridha Ati Jaafar

Keywords:

melanoidin
fluorescence spectroscopy
hydrogen peroxide
cells

Abstract

Possible identify and describe a polymer formed from Glc with Ala by Fluorescence spectroscopy. Melanoidins before dialysis was higher than those of melanoidin after dialysis. Time‐resolved fluorescence has been applied in the characterization of melanoidin and was observed. Melanoidins show a decrease in the absorption after the addition of hydrogen peroxide when the concentrations of H2O2 from 100 - 200 μl are used. However, the data suggests that the melanoidin did not penetrate into the cells (CHO-K1) after 1 and 2 hours, but it can be observed after 24 hours.

Abbreviation: TRES, time-resolved emission spectra ; Glc, Glucose ; Ala, Alanine ; YFP, Yellow fluorescence protein ; GFP, green fluorescence protein ; CHO, Chinese hamster ovary

Author Biographies

Ghassan Faisal Mohsin

General Directorate Vocational Education | Ministry of Education | Iraq

Abdulridha Ati Jaafar

Faculty of Agriculture | University of Misan | Iraq

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Published

2020-03-30

How to Cite

1.
Characterization of melanoidin formed from glucose with alanine  by Fluorescence spectroscopy and redox chemistry in vitro cells. JNSLAS [Internet]. 2020 Mar. 30 [cited 2024 Aug. 25];4(1):29-17. Available from: https://journals.ajsrp.com/index.php/jnslas/article/view/2250

Issue

Vol. 4 No. 1 (2020)

Section

Content

License

Copyright (c) 2020 المجلة العربية للعلوم ونشر الأبحاث

How to Cite

1.
Characterization of melanoidin formed from glucose with alanine  by Fluorescence spectroscopy and redox chemistry in vitro cells. JNSLAS [Internet]. 2020 Mar. 30 [cited 2024 Aug. 25];4(1):29-17. Available from: https://journals.ajsrp.com/index.php/jnslas/article/view/2250